Recombinant Human sFas Ligand

Synonyms :

soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL

Description :

Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.

Catalog #:

310-03H

Source :

CHO cells

Purity :

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED₅₀ for this effect is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁵ units/mg.

AA Sequence :

HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

PubMed Link :

sFas Ligand

Country of Origin :

USA

Size A	2µg	$80
Size B	10µg	$195
Size C	1mg	$4300

Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.

Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.