Anti-Human ApoE3*

Preparation :

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Anti-Human ApoE3 specific antibody was purified by affinity chromatography employing immobilized hApoE3 matrix. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA

Catalog #:

500-P238

Source :

Rabbits

Formulation :

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Stability :

The lyophilized antibody is stable for at least 2 years from date of receipt at -20⁰C. The reconstituted antibody is stable for at least two weeks at 2-8⁰C. Frozen aliquots are stable for at least 6 months when stored at -20⁰C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Applications :

Elisa 

Indirect: To detect hApoE3 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.
Sandwich: To detect hApoE3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human ApoE3 (500-P238Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Western Blot 

To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Country of Origin :

USA

Size A	50µg	$195
Size B	100µg	$250
Size C	1mg	$2100

Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.

Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.