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Animal-Free Recombinant Human sTNF Receptor Type I

Product Details

Catalog Number: AF-310-07
Description:

TNFRI belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TNF-α or TNF-β to TNFRI initiates a signal transduction pathway that results in the activation of the transduction factor NF-κB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. Soluble TNF Receptor I (sTNFRI) is capable of inhibiting TNF-α and TNF-β activities by acting as a decoy receptor that serves as a sink for the TNF ligands. The human TNFRI gene encodes for a 455 amino acid type I transmembrane protein, which contains a 21 amino acid signal sequence, a 190 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 221 amino acid cytoplasmic domain. Recombinant Human sTNF Receptor Type I is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine-rich, ligand-binding portion of the extracellular domain of the TNFRI protein.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: soluble Tumor Necrosis Factor Type I, TNFRSF1A, TNFAR, p60, p55, CD120a, TNFR1

AA Sequence: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The  ED50  for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.05 µg/ml.

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.