Monoclonal Antibodies

Anti-Human Adiponectin (Monoclonal Mouse)

Product Details

Catalog Number: 500-M126

Source: Monoclonal Mouse

Preparation: Produced in Balb/c mice using highly pure recombinant Human Adiponectin as the immunizing antigen.  This antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: Hi-5 Insect cells derived Recombinant Human Adiponectin (PeproTech catalog# 450-24)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/ml.

Anti-Human Adiponectin Sandwich ELISA

Western Blot: To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.

Anti-Human Adiponectin Western Blot Unreduced Anti-Human Adiponectin Western Blot Reduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.