Monoclonal Antibodies

Anti-Human BAFF (Monoclonal Mouse)

Product Details

Catalog Number: 500-M64

Source: Monoclonal Mouse

Preparation: Produced in BALB/c mice using highly pure (>98%) recombinant human BAFF as the immunizing antigen. This IgG1κ antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: E.coli derived Recombinant Human BAFF (PeproTech catalog# 310-13)

Sandwich ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 5.0-6.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human BAFF when used with PeproTech's biotinylated antigen affinity purified anti-human BAFF (500-P163GBt) as the detection antibody at a concentration of approximately 0.25-0.50 µg/ml.

Anti-Human BAFF Sandwich ELISA

Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

Anti-Human BAFF Western Blot Reduced Anti-Human BAFF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Human
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.