Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-4 (human Beta Defensin-4). Anti-Human BD-4 specific antibody was purified by affinity chromatography employing immobilized hBD-4 matrix.
E.coli derived Recombinant Human BD-4 (PeproTech catalog# 300-65)
To detect hBD-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BD-4 (500-P268Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-4.
To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.