Produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
HeLa cells derived Recombinant Human BMP-4 (PeproTech catalog# 120-05)
In a sandwich ELISA (assuming100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 2000 pg/ml of Recombinant Human BMP-4 when used in conjunction with compatible secondary reagents.
To detect Human BMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.