Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Eotaxin (CCL11) (Polyclonal Goat)

Product Details

Catalog Number: 500-P41G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography employing immobilized hEotaxin matrix.

Immunogen: E.coli derived Recombinant Human Eotaxin (CCL11) (PeproTech catalog# 300-21)

Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Eotaxin (500-P41GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin.

Anti-Human Eotaxin (CCL11) Sandwich ELISA

Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Eotaxin (CCL11) Western Blot Reduced Anti-Human Eotaxin (CCL11) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.