Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
E.coli derived Recombinant Human FGF-basic (154 a.a.) (PeproTech catalog# 100-18B)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human FGF-basic when used with PeproTech's biotinylated antigen affinity purified anti-human FGF-basic (500-P18Bt) as the detection antibody at a concentration of approximately 0.125-0.25 µg/ml.
To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.