Antigen Affinity Purified Polyclonal Antibodies

Anti-Human FGF-basic

Product Details

Catalog Number: 500-P18

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human FGF-basic. Anti-Human FGF-basic specific antibody was purified by affinity chromatography employing immobilized Human FGF-basic matrix.

Immunogen: E.coli derived Recombinant Human FGF-basic (154 a.a.) (PeproTech catalog# 100-18B)

Sandwich ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human FGF-basic (500-P18Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-basic.

Anti-Human FGF-basic Sandwich ELISA

Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human FGF-basic  Western Blot Reduced Anti-Human FGF-basic  Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Human, Human + Rat, N/A
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.