Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Flt3-Ligand

Product Details

Catalog Number: 500-P42

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt-3 Ligand. Anti-Human Flt-3 Ligand specific antibody was purified by affinity chromatography employing immobilized hFlt-3 Ligand matrix.

Immunogen: E.coli derived Recombinant Human Flt3-Ligand (PeproTech catalog# 300-19)

Sandwich ELISA: To detect hFlt-3 Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Flt-3 Ligand (500-P42Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.

Anti-Human Flt3-Ligand Sandwich ELISA

Western Blot: To detect hFlt-3 Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt-3 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Flt3-Ligand Western Blot Reduced Anti-Human Flt3-Ligand Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.