Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Anti-Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.
E.coli derived Recombinant Human Follistatin (PeproTech catalog# 120-13)
To detect Human Follistatin by sandwich ELISA
(using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this
antibody is required. This antigen affinity purified antibody, in conjunction
with PeproTech’s Biotinylated Anti-Human Follistatin (500-P207Bt) as a
detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of
recombinant Human Follistatin.
To detect Human Follistatin by Western Blot
analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible
secondary reagents the detection limit for recombinant Human Follistatin is 1.5
- 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.