Produced in Balb/c mice using highly pure Recombinant Human G-CSF as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Animal-Free Recombinant Human G-CSF (PeproTech catalog# AF-300-23)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of Recombinant Human G-CSF when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human G-CSF (500-P43Bt) as the detection antibody, at a concentration of approximately 0.5-1.0 μg/ml.
To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.