Monoclonal Antibodies

Anti-Human G-CSF (Monoclonal Mouse)

Product Details

Catalog Number: 500-M37

Source: Monoclonal Mouse

Preparation:

Produced in Balb/c mice using highly pure Recombinant Human G-CSF as the immunizing antigen.  This antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: E.coli derived Animal-Free Recombinant Human G-CSF (PeproTech catalog# AF-300-23)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human G-CSF (500-P43BT) as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.

Anti-Human G-CSF Sandwich ELISA

Western Blot: To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.

Anti-Human G-CSF Western Blot Reduced Anti-Human G-CSF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.