Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IFN-α (Polyclonal Goat)

Product Details

Catalog Number: 500-P32AG

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure recombinant Human IFN-α. Anti-Human IFN-α specific antibody was purified by affinity chromatography employing immobilized Human IFN-α matrix.

Immunogen: E.coli derived Recombinant Human IFN-α (PeproTech catalog# 300-02A)*
*Catalog# 300-02A has been replaced with 300-02AA.

Sandwich ELISA: To detect Human IFN-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IFN-α (500-P32AGBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-α.

Anti-Human IFN-α Sandwich ELISA

Western Blot: To detect Human IFN-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IFN-α Western Blot Reduced Anti-Human IFN-α Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.


Due to patent, this product is not available to all customers. Please contact your local sales office for further information.

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.