Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IGF-BP1

Product Details

Catalog Number: 500-P228

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Anti-Human IGF-BP1 specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP1 matrix.

Immunogen: E.coli derived Recombinant Human IGF-BP1 (PeproTech catalog# 350-10)

Sandwich ELISA: To detect hIGF-BP1 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IGF-BP1 (500-P228Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.

Anti-Human IGF-BP1 Sandwich ELISA

Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IGF-BP1 Western Blot Reduced Anti-Human IGF-BP1 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.