Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.
E.coli derived Recombinant Human IL-10 (PeproTech catalog# 200-10)
In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human IL-10 (500-P20BT) as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.
To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.