Anti-Human IL-12 (CHO-derived immunogen) 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P154G Description: Source: Polyclonal Goat Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix. Immunogen: CHO cells derived Recombinant Human IL-12 p70 (PeproTech catalog# 200-12) Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-12 (500-P154GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Cancer Immune System Inflammation Stem Cells & Differentiation Allergy Transplantation Recent Citations First Author Scott, T A Title Broadly active zinc finger protein-guided transcriptional activation of HIV-1. Citation Molecular therapy. Methods & clinical development; 20 pg18-29 PubMed ID 33335944 First Author Bouhaddou, M Title The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Citation Cell; 182(3) pg685-712.e19 PubMed ID 32645325 First Author Woo, J S Title CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses. Citation Journal of immunology (Baltimore, Md. : 1950); 201(4) pg1174-1185 PubMed ID 29987160