Monoclonal Antibodies

Anti-Human IL-6 (Monoclonal Mouse)

Product Details

Catalog Number: 500-M06

Source: Monoclonal Mouse

Preparation: Produced in BALB/c mice using highly pure (>98%) recombinant human IL-6 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: E.coli derived Recombinant Human IL-6 (PeproTech catalog# 200-06)

Sandwich ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 800 pg/ml of recombinant human IL-6 when used with PeproTech's biotinylated antigen affinity purified anti-human IL-6 (500-P26Bt) as the detection antibody at a concentration of approximately 0.1-0.2 µg/ml.

Anti-Human IL-6 Sandwich ELISA

Western Blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 3.5-4.0 ng/lane, under reducing conditions and 1.5-2.0 ng/lane, under non-reducing conditions.

Anti-Human IL-6 Western Blot Reduced Anti-Human IL-6 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Human
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.