Produced from sera of rabbits pre-immunized with highly pure recombinant Human Klotho. Anti-Human Klotho specific antibody was purified by affinity chromatography employing immobilized Human Klotho matrix.
E.coli derived Recombinant Human Klotho (PeproTech catalog# 100-53)
To detect hKlotho by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Klotho (500-P296Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKlotho.
To detect hKlotho by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKlotho is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.