Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MCP-1 (CCL2)

Product Details

Catalog Number: 500-P34

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF). Anti-Human MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized hMCP-1(MCAF) matrix.

Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04)

Sandwich ELISA: To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-1(MCAF) (500-P34Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF).

Anti-Human MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MCP-1 (CCL2) Western Blot Reduced Anti-Human MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Human, Mouse
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.