Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human MCP-4 (CCL13) (PeproTech catalog# 300-24)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human MCP-4 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.