Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MIA-2

Product Details

Catalog Number: 500-P255

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA-2 (human MIA-2). Anti-Human MIA-2 specific antibody was purified by affinity chromatography employing immobilized hMIA-2 matrix.

Immunogen: E.coli derived Recombinant Human MIA-2 (PeproTech catalog# 130-02)

Sandwich ELISA: To detect hMIA-2 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MIA-2 (500-P255Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.

Anti-Human MIA-2 Sandwich ELISA

Western Blot: To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MIA-2 Western Blot Reduced Anti-Human MIA-2 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.