Produced in mice using highly pure recombinant human β-NGF as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human β-NGF (PeproTech catalog# 450-01)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 200 pg/ml of recombinant Human β-NGF when used with PeproTech's biotinylated antigen affinity purified anti-Human β-NGF (500-P85Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human β-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.