Monoclonal Antibodies

Anti-Human β-NGF (Monoclonal Mouse)

Product Details

Catalog Number: 500-M85

Source: Monoclonal Mouse

Preparation: Produced in mice using highly pure recombinant human β-NGF as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: E.coli derived Recombinant Human β-NGF (PeproTech catalog# 450-01)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 200pg/ml of Recombinant Human β-NGF when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human β-NGF (500-P85BT) as the detection antibody at a concentration of approximately 0.5- 1.0 μg/ml.

Anti-Human β-NGF Sandwich ELISA

Western Blot: To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human β-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Anti-Human β-NGF Western Blot Unreduced Anti-Human β-NGF Western Blot Reduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.