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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human β-NGF

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Product Details

Catalog Number: 500-P85
Description:
Anti-Human β-NGF
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human β-NGF. Anti-Human β-NGF specific antibody was purified by affinity chromatography employing immobilized Human β-NGF matrix.

Immunogen: E.coli derived Recombinant Human β-NGF (PeproTech catalog# 450-01)

Sandwich ELISA: To detect Human β-NGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human β-NGF (500-P85Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human β-NGF.

Anti-Human β-NGF Sandwich ELISA

Western Blot: To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human β-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human β-NGF Western Blot Reduced Anti-Human β-NGF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Recent Citations

First Author
Peng, T
Title
Keratinocytes produce IL-17c to protect peripheral nervous systems during human HSV-2 reactivation.
Citation
The Journal of Experimental Medicine; 214(8) pg2315-2329
PubMed ID
First Author
Tsunoda, S
Title
Significance of nerve growth factor overexpression and its autocrine loop in oesophageal squamous cell carcinoma.
Citation
British Journal of Cancer; 95(3) pg322-30
PubMed ID
First Author
Miknyoczki, S J
Title
The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice.
Citation
Clinical cancer research : an official journal of the American Association for Cancer Research; 8(6) pg1924-31
PubMed ID