Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human sFas Receptor. Anti-Human sFas Receptor specific antibody was purified by affinity chromatography employing an immobilized Human sFas Receptor matrix.

Immunogen: E.coli derived Recombinant Human sFas Receptor (PeproTech catalog# 310-20)

Sandwich ELISA: To detect Human sFas Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sFas Receptor (500-P295Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human sFas Receptor.

Western Blot: To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sFas Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.