Produced in BALB/c mice immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human sTRAIL/Apo2L (PeproTech catalog# 310-04)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500pg/ml of Recombinant Human TRAIL/Apo2L when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human TRAIL/Apo2L (500-P135BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
To detect Human TRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.