Antigen Affinity Purified Polyclonal Antibodies

Anti-Human TPO (Polyclonal Rabbit)

Product Details

Catalog Number: 500-P49

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

Immunogen: E.coli derived Animal-Free Recombinant Human TPO (PeproTech catalog# AF-300-18)

Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TPO (500-P49Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Anti-Human TPO Sandwich ELISA

Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human TPO Western Blot Reduced Anti-Human TPO Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.