Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-2 as the immunizing antigen. This IgG2Aκ antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Recombinant Murine IL-2 (PeproTech catalog# 212-12)
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Murine IL-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Murine IL-2 (500-P111BT) as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.
To detect Murine IL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.