Monoclonal Antibodies

Anti-Murine IL-2 (Monoclonal Rat)

Product Details

Catalog Number: 500-M127

Source: Monoclonal Rat

Preparation: Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-2 as the immunizing antigen. This IgG2Aκ antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: E.coli derived Recombinant Murine IL-2 (PeproTech catalog# 212-12)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Murine IL-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Murine IL-2 (500-P111BT) as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.

Anti-Murine IL-2 Sandwich ELISA

Western Blot: To detect Murine IL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.

Anti-Murine IL-2 Western Blot Reduced Anti-Murine IL-2 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.