Produced in BALB/c mice using highly pure (>98%) recombinant rat IL-2 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Rat IL-2 (PeproTech catalog# 400-02)
In a sandwich ELISA (assuming 100μl/well), a concentration of 14.0-16.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Rat IL-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Rat IL-2 (500-P274BT) as the detection antibody at a concentration of approximately 0.10-0.15 μg/ml.
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.