Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat MCP-1 (CCL2)

Product Details

Catalog Number: 500-P76

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1(MCAF). Anti-Rat MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1(MCAF) matrix.

Immunogen: E.coli derived Recombinant Rat MCP-1 (CCL2) (PeproTech catalog# 400-12)

Sandwich ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat MCP-1(MCAF) (500-P76Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).

Anti-Rat MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat MCP-1 (CCL2) Western Blot Reduced Anti-Rat MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity:
Rat
Research Interest

Country Of Origin: USA

Not for human use.