Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Anti-Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Human 4-1BB Ligand (PeproTech catalog# 310-11)
To detect h4-1BBL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human 4-1BBL (500-P169) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant h4-1BBL.
To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.