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Biotinylated Anti-Human Amphiregulin

Product Details

Catalog Number: 500-P322BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human Amphiregulin (PeproTech catalog# 100-55B)

Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Amphiregulin (500-P322) as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin.

Biotinylated Anti-Human Amphiregulin Sandwich ELISA

Western Blot: To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human Amphiregulin Western Blot Unreduced Biotinylated Anti-Human Amphiregulin Western Blot Reduced

Note:

1mg will be provided as 2x500μg.

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.