Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human FGF-basic

Product Details

Catalog Number: 500-P18Bt

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-basic. Anti- Human FGF-basic specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human FGF-basic (154 a.a.) (PeproTech catalog# 100-18B)

Sandwich ELISA: To detect hFGF-basic by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human FGF-basic (500-P18) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hFGF-basic.

Biotinylated Anti-Human FGF-basic Sandwich ELISA

Western Blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human FGF-basic Western Blot Unreduced Biotinylated Anti-Human FGF-basic Western Blot Reduced

Note:

1mg will be provided as 2x500μg.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.