Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Anti-Human Follistatin specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Human Follistatin (PeproTech catalog# 120-13)
To detect Human Follistatin by sandwich ELISA
(using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of
this antibody is required. This biotinylated polyclonal antibody, in
conjunction with PeproTech’s Polyclonal Anti- Human Follistatin (500-P207) as a
capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of
recombinant Human Follistatin.
To detect Human Follistatin by Western Blot
analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible
development reagents the detection limit for recombinant Human Follistatin is
1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.