Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17E. Anti-Human IL-17E specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Human IL-17E (PeproTech catalog# 200-24)
To detect Human IL-17E by sandwich ELISA (using
100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this
antibody is required. This antigen affinity purified antibody, in conjunction
with PeproTech’s Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection
antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human
To detect Human IL-17E by Western Blot analysis
this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible
secondary reagents the detection limit for recombinant Human IL-17E is 1.5 -
3.0 ng/lane, under either reducing or non-reducing conditions.