Produced from sera of rabbits immunized with
highly pure recombinant Human IL-36γ. Anti-Human IL-36γ-specific antibody was
purified by affinity chromatography and then biotinylated.
E.coli derived Animal-Free Recombinant Human IL-36γ (IL-1F9) (PeproTech catalog# AF-200-36G)
To detect Human IL-36γ by sandwich ELISA (using
100 ml/well), a concentration of 0.25 – 1.0 μg/ml of
this antibody is required. This biotinylated polyclonal antibody, in
conjunction with PeproTech’s Polyclonal Anti- Human IL-36γ (500-P316) as a
capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant
To detect Human IL-36γ by Western Blot analysis,
this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible
development reagents, the detection limit for recombinant Human IL-36γ is 1.5 –
3.0 ng/lane, under either reducing or non-reducing conditions.