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Biotinylated Anti-Human VEGF-B

Product Details

Catalog Number: 500-P267BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B. Anti-Human VEGF-B-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human VEGF-B (PeproTech catalog# 100-20B)

Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human VEGF-B (500-P267) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human VEGF-B.

 Biotinylated Anti-Human VEGF-B Sandwich ELISA

Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

 Biotinylated Anti-Human VEGF-B Western Blot Reduced  Biotinylated Anti-Human VEGF-B Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.