Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human WNT-3a

Product Details

Catalog Number: 500-P251BT

Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich ELISA: To detect hWNT-3a by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human WNT-3a (500-P251) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hWNT-3a.

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Anti-Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.

Source: Polyclonal Rabbit

Research Interest

Country Of Origin: USA

Not for human use.