Biotinylated Polyclonal Antibodies

Biotinylated Anti-Murine IL-1β

Product Details

Catalog Number: 500-P51BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1β. Anti-Murine IL-1β specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine IL-1β (PeproTech catalog# 211-11B)

Sandwich ELISA: To detect mIL-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-1β (500-P51) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-1β.

Biotinylated Anti-Murine IL-1β Sandwich ELISA

Western Blot: To detect mIL-1β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Murine IL-1β Western Blot Reduced Biotinylated Anti-Murine IL-1β Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

Cross Reactivity:
Mouse, Mouse + Bacteria
Research Interest

Country Of Origin: USA

Not for human use.