Biotinylated Anti-Murine IL-15 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P173BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Anti-Murine IL-15 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Murine IL-15 (PeproTech catalog# 210-15) Sandwich ELISA: To detect mIL-15 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-15 (500-P173) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-15. Western Blot: To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Apoptosis Cancer Cell Culture Immune System Inflammation Stem Cells & Differentiation Recent Citations First Author Anthony, S M Title Inflammatory Signals Regulate IL-15 in Response to Lymphodepletion. Citation Journal of immunology (Baltimore, Md. : 1950); 196(11) pg4544-52 PubMed ID 27183627 First Author Polansky, J K Title High dose CD11c-driven IL15 is sufficient to drive NK cell maturation and anti-tumor activity in a trans-presentation independent manner. Citation Scientific Reports; 6 pg19699 PubMed ID 26822794 First Author Colpitts, S L Title Transcriptional regulation of IL-15 expression during hematopoiesis. Citation Journal of immunology (Baltimore, Md. : 1950); 191(6) pg3017-24 PubMed ID 23966624
