Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Anti-Murine IL-5 specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Murine IL-5 (PeproTech catalog# 215-15)
To detect Murine IL-5 by sandwich ELISA (using
100 ml/well) a concentration of 0.25 – 1.0 μg/ml of
this antibody is required. This biotinylated polyclonal antibody, in
conjunction with PeproTech’s Polyclonal Anti- Murine IL-5 (500-P55) as a
capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of
recombinant Murine IL-5.
To detect Murine IL-5 by Western Blot analysis
this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible
development reagents, the detection limit for recombinant Murine IL-5 is 1.5 –
3.0 ng/lane, under either reducing or non-reducing conditions.