Biotinylated Polyclonal Antibodies

Biotinylated Anti-Murine IP-10 (CXCL10)

Product Details

Catalog Number: 500-P129BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIP-10. Anti-Murine IP-10 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine IP-10 (CXCL10) (PeproTech catalog# 250-16)

Sandwich ELISA: To detect mIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IP-10 (500-P129) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIP-10.

Western Blot: To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Cross Reactivity:
Mouse
Research Interest

Country Of Origin: USA

Not for human use.