Produced from sera of rabbits immunized with highly pure Recombinant Murine LIGHT. Anti-Murine LIGHT-specific antibody was purified by affinity chromatography and then biotinylated.
E.coli-derived Recombinant Murine LIGHT (PeproTech catalog# 315-12)
To detect Murine LIGHT by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine LIGHT (500-P308) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Murine LIGHT.
To detect Murine LIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.