Anti-Human GM-CSF 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P33 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix. Immunogen: E.coli derived Recombinant Human GM-CSF (PeproTech catalog# 300-03) Sandwich ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human GM-CSF (500-P33Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Cancer Cell Culture Inflammation Proliferation Stem Cells & Differentiation Wound Healing Recent Citations First Author Bialek-Waldmann, J K Title Induced dendritic cells co-expressing GM-CSF/IFN-α/tWT1 priming T and B cells and automated manufacturing to boost GvL. Citation Molecular therapy. Methods & clinical development; 21 pg621-641 PubMed ID 34095345 First Author Kim, S Title A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer. Citation Molecular therapy. Methods & clinical development; 2 pg15031 PubMed ID 26445724 First Author Chong, H C Title Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing. Citation The Journal of Cell Biology; 184(6) pg817-31 PubMed ID 19307598
