Anti-Human IL-10 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P20 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-10. Anti-Human IL-10 specific antibody was purified by affinity chromatography employing immobilized hIL-10 matrix. Immunogen: E.coli derived Recombinant Human IL-10 (PeproTech catalog# 200-10) Sandwich ELISA: To detect hIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-10 (500-P20Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Immune System Inflammation Neurobiology Stem Cells & Differentiation Allergy Transplantation Recent Citations First Author Oliveira, J A Title Mycobacterium leprae induces a tolerogenic profile in monocyte-derived dendritic cells via TLR2 induction of IDO. Citation Journal of Leukocyte Biology; 110(1) pg167-176 PubMed ID 33040382 First Author O'Connor, M H Title A follicular regulatory Innate Lymphoid Cell population impairs interactions between germinal center Tfh and B cells. Citation Communications Biology; 4(1) pg563 PubMed ID 33980982 First Author Bouhaddou, M Title The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Citation Cell; 182(3) pg685-712.e19 PubMed ID 32645325