Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor α. Anti-Human sIL-2 Receptor α-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor α matrix.

Immunogen: CHO cells derived Recombinant Human IL-2 Receptor α (PeproTech catalog# 200-02RC)

Sandwich ELISA: To detect Human sIL-2 Receptor α by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sIL-2 Receptor α (500-P22RBT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor α.

Western Blot: To detect Human sIL-2 Receptor α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sIL-2 Receptor α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.