Anti-Murine IL-2 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P111 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-2. Anti-Murine IL-2 specific antibody was purified by affinity chromatography employing immobilized mIL-2 matrix. Immunogen: E.coli derived Recombinant Murine IL-2 (PeproTech catalog# 212-12) Sandwich ELISA: To detect mIL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-2 (500-P111Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-2. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Cell Culture Diabetes/Weight Regulation Immune System Inflammation Neurobiology Proliferation Stem Cells & Differentiation Wound Healing Allergy Transplantation Recent Citations First Author Polesso, F Title Constitutive expression of NF-κB inducing kinase in regulatory T cells impairs suppressive function and promotes instability and pro-inflammatory cytokine production. Citation Scientific Reports; 7(1) pg14779 PubMed ID 29116141 First Author Pérol, L Title Loss of immune tolerance to IL-2 in type 1 diabetes. Citation Nature Communications; 7 pg13027 PubMed ID 27708334 First Author Okoye, I S Title Transcriptomics identified a critical role for Th2 cell-intrinsic miR-155 in mediating allergy and antihelminth immunity. Citation Proceedings of the National Academy of Sciences of the United States of America; 111(30) pgE3081-90 PubMed ID 25024218