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Protocols - Immunohistochemistry: Mouse Anti-Human sIL-2R

The following protocol used human frozen tonsil tissue.

  1. Incubate the tissue section for 10 minutes in acetone.
  2. Incubate the tissue section for 20 minutes at room temperature with Mouse Anti-Human sIL-2R at 5.0 μg/mL. Wash the slide twice for three minutes each.
  3. Incubate the tissue section for 10 minutes with a peroxidase block.  Wash the slide twice for three minutes each.
  4. Incubate the tissue section for 20 minutes with a labeled polymer. Wash the slide twice for three minutes each.
  5. Incubate the tissue section for up to 10 minutes with DAB.
  6. Counterstain the tissue section with hematoxylin. 

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