Animal-Free Recombinant Human GM-CSF 1 ReviewsSubmit a Review Product Details Catalog Number: AF-300-03 Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α AA Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 14.6 kDa Accession Number: P04141 Gene ID: 1437 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Cancer Cell Culture Immune System Inflammation Stem Cells & Differentiation Wound Healing Recent Citations First Author Creelan, B C Title Tumor-infiltrating lymphocyte treatment for anti-PD-1-resistant metastatic lung cancer: a phase 1 trial Citation Nature Medicine; 27(8) pg1410-1418 PubMed ID 34385708 First Author Rodríguez-Jiménez, P Title Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis Citation Scientific Reports; 11(1) pg14579 PubMed ID 34272424 First Author Schulte, M Title The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress Citation Communications Biology; 4(1) pg520 PubMed ID 33947954 Product Reviews Existing reviews Very good experience We use GM-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 8/19/2020 × Very good experience We use GM-CSF to differentiate human and rabbit macrophages and we can highly recommend it. From: Hanna-Mari | Date: 8/19/2020 Close
