Recombinant Human LIF 0 ReviewsSubmit a Review Product Details Catalog Number: 300-05 Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds. Source: E.coli Synonyms: Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Interleukin 6 family cytokine AA Sequence: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 19.7 kDa Accession Number: P15018 Gene ID: 3976 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Mouse, Pig, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Diabetes/Weight Regulation Immune System Lipid Metabolism Neurobiology Stem Cells & Differentiation Recent Citations First Author Zhi, M Title Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Citation Cell Research; PubMed ID 34848870 First Author Kobayashi, T Title Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells. Citation Cell Reports; 37(2) pg109812 PubMed ID 34644585 First Author Sozen, B Title Reconstructing aspects of human embryogenesis with pluripotent stem cells Citation Nature Communications; 12(1) pg5550 PubMed ID 34548496
